Oleanolic Acid Improved Inflammatory Response and Apoptosis of PC12 Cells Induced by OGD/R Through Downregulating miR-142-5P

Background Oleanolic acid (OA) has notable anti-inflammatory and anti-tumor effects, but the role of OA in cerebral ischemia-reperfusion injury (CIRI) not been reported so far. Methods Oxygen glucose deprivation/reoxygenation (OGD/R) model was induced PC12 cells. MTT assay used to detect cell viability cells, while ELISA detected expression TNF-?, IL-1? IL-6. The superoxide dismutase (SOD), malondialdehyde (MDA) reactive oxygen species (ROS) by appropriate kits, apoptosis Tunel technique. Western blot apoptosis-related proteins. transfection technique overexpressed miR-142?5p. After overexpressing miR-142?5p technique, RT-qPCR. Results Besides ability promote acitivity, ameliorated OGD/R-induced inflammatory response Moreover, capability alleviate inflammation cells observed be related down-regulation Conclusion improved OGD/R through downregulating miR-142?5P